Quantum dot test paper capable of quantitatively detecting florfenicol residues in various matrixes

• Main Authors: LU FEI, XIAO YOUYU
• Format: Patent
• Language: Chinese; English
• Published: 10.04.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses quantum dot test paper capable of quantitatively detecting florfenicol residues in various matrixes. The test paper is prepared by sequentially adhering a sample pad, a markerpad, a chromatographic membrane and a buffer pad to a PVC bottom plate in a 1-2mm overlapping manner, the chromatographic membrane is a nitrocellulose membrane carrying a detection line and a qualitycontrol line, and the test paper is characterized in that the detection line is coated with a florfenicol antigen, and the quality control line is coated with a DNP monoclonal antibody, and the markerpad is loaded with quantum dots coupled with a florfenicol antibody and DNP-BSA. The quantum dot test paper provided by the invention can realize direct sample loading detection of dairy product rawmaterials without pretreatment, meat product eggs are simply crushed and then loaded after being treated by a certain amount of methanol solvent, the pretreatment is simple, and the matrix benefit issmall. 本发明公开了一种可定量检测多种基质中氟苯尼考