Application of PLEKHG5 gene methylation in asthenospermia diagnostic agent and kit

• Main Authors: XUE XINGKUI, GU YANLI, DU YE, CHEN XIAOLING, MA XIAORUI
• Format: Patent
• Language: Chinese; English
• Published: 21.01.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to application of the methylation level of a human PLEKHG5 gene promoter region in preparation of a detection agent for detecting sperm motility. The human PLEKHG5 gene promoterregion is located in a region corresponding to 6545514-6548214 sites of human No.1 chromosome. Analysis and study based on whole-genome methylation sequencing results and verification find that whether part of methylation sites of the human PLEKHG5 gene promoter region are methylated has significance difference between asthenospermia patients and healthy people, which can be used as a molecular marker to diagnose whether subjects suffer from asthenospermia. When all methylation sites of the region are not methylated, the possibility that subjects suffer from the asthenospermia is larger. The molecular marker can provide auxiliary evaluation information for diagnosis of the asthenospermia and identify whether the subjects suffer from the asthenospermia with other diagnosis indexes. 本发明涉及人PLEKHG5基因启动子区的甲基化水平在制备检测精子活