Preparation method and use method of genetically engineered nucleic acid factor paste capable of delaying skin aging

• Main Authors: WANG XIUFANG, LIU SHUFENG, SU LIBO, LYU BAIXUE, JI NING, SONG ZHIXUE, YAN LIFANG, LYU ZHANJUN
• Format: Patent
• Language: Chinese; English
• Published: 10.12.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a preparation method and a use method of genetically engineered nucleic acid factor paste capable of delaying skin aging. The preparation method of the genetically engineered nucleic acid factor paste capable of delaying skin aging comprises the following steps: step one, preparing genetically engineered small-molecule RNA without endotoxin contamination, namely to constructexpression vector by inserting DNA sequence expressing human small RNA into pET-28-alpha plasmid, transform BL-21 bacterium with the expression vector, produce human small RNA with the transformed BL-21 bacterium by adopting IPTG induction and extract human small RNA without endotoxin contamination by using SDS-NaCl; and step two, preparing the genetically engineered nucleic acid factor paste capable of delaying skin aging, namely to dry the prepared human small RNA, mix the dried RNA with a matrix and add a transdermal absorbent so as to obtain the genetically engineered nucleic acid factorpaste capable of delaying