Method for separating and purifying prawn viscera chitin excision enzymes and application of prawn viscera chitin excision enzymes

• Main Authors: WANG LINGLING, ZHENG ZHIFU, SUN LIDAN, XIE XIAOLAN, LYU FENGJIAO
• Format: Patent
• Language: Chinese; English
• Published: 29.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biology, and specifically discloses a method for separating and purifying prawn viscera chitin excision enzymes and application of the prawn viscera chitin excision enzymes. The method comprises the steps: adopting prawn viscera as a raw material, performing grinding, performing extraction by using a Tris-HC1 buffer solution so as to obtain a chitinexcision enzyme crude extract, then performing ammonium sulfate fractional separation, performing dialysis for salt removal, and performing purification through gel column chromatography and anion exchange column chromatography so as to obtain the chitin excision enzymes. The chitin excision enzymes obtained through separation and purification have a certain inhibitory effect on both Escherichia coli and Staphylococcus aureus, and a foundation for researches, development and production in further utilization of the chitin excision enzymes is laid; and in addition, the chitin excision enzymes in prawn viscera are devel