Development method and application of molecular marker for rapid screening of red pulp trait of malus domestica

• Main Authors: SHA GUANGYA, LI PENGMIN, ZOU YANGJUN, MA FENGWANG, WEI TAO, XU LINGFEI, LI CUIYING, YANG CHENGQUAN
• Format: Patent
• Language: Chinese; English
• Published: 22.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a development method and application of a molecular marker for rapid screening of the red pulp trait of malus domestica, and belongs to the technical field of agriculture. According to the development method and application of the molecular marker for rapid screening of the red pulp trait of the malus domestica, the F1 isolated populations of varieties of red 3* changfu No.2 of which the promoter sequences of the MYB10 gene are R6R6 and R1R1 respectively are used as research objects, the anthocyanin contents of the ripe pulp of the varieties are determined, a genetic linkage map is constructed, and candidate genes which control the pulp shade trait are identified. The development method and application of the molecular marker for rapid screening of the red pulp trait of the malus domestica belongs to marker-assisted breeding; by using the molecular marker for early screening of the red pulp trait, time, manpower and land resources can be greatly saved, the breeding efficiency can be gr