Rapid detection of Escherichia coli in thin film culture device

• Main Authors: SADDORIS HALEY A, CHANDRAPATI SAILAJA, TEAGARDEN ALEC J
• Format: Patent
• Language: Chinese; English
• Published: 19.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a [beta]-D-glucuronidase indicator system, and a first cold-water soluble gelling agentadhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. The first sheet and second sheet are configured to retard passage of carbon dioxide therethrough. Methods of using the device are also provided. 本发明提供一种用于差分计数大肠菌群和大肠杆菌(Escherichia coli)微生物的菌落的装置。所述装置包括不透水的第一片；不透水的第二片，所述第二片附接到所述第一片；干燥的可再水合培养基，所述培养基包含乳糖发酵指示剂体系、β-D-葡糖苷酸酶指示剂体系和粘附到所述第一片的第一冷水可溶性胶凝剂，所述培养基设置在微生物生长区中；和第二冷水可溶性胶凝剂，所述第二冷水可溶性胶凝剂粘附到所述第二片。所述微生物生长区设置在所述第一片和所述第二片之间。所述第一片和所述第二片被构