Non-enzyme method full-nucleic acid extracting kit

• Main Authors: JIEKUN LI, YU ZHANG, DONGYU FAN, LUFENG REN, JING GAO, YIMEI CAI, YOULAI DAI
• Format: Patent
• Language: Chinese; English
• Published: 15.11.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of molecular biology, and particularly relates to a non-enzyme method full-nucleic acid extracting kit for the aspect of bacterium full-nucleic acid extraction and purification. The kit is characterized by comprising a lysate, a combining solution, a magnetic bead solution, a washing solution A, a washing solution B and a dissolution solution. Thallus cells are split through the lysate, nucleic acid is released for capturing magnetic beads, no enzyme is needed in the whole extracting process, complete nucleic molecules can be extracted, the purity is high, the kit can be directly applied to downstream biology experiments, the extracting process is rapid and nontoxic, operation is easy, and the kit is used in cooperation with an automatic instrument. 本发明属于分子生物学领域，尤其涉及一种细菌全核酸提取纯化方面，是一种无酶法全核酸提取试剂盒，其特征在于试剂盒包括：裂解液、结合液、磁珠液、洗涤液A、洗涤液B、溶解液。通过裂解液裂解菌体细胞，释放核酸被磁珠捕捉，整个提取过程无需酶试剂，可以提取完整的核酸分子，纯度高，可直接应用下游生物实验，提取过程快速、无毒、操作简单，并可配合自动化仪器使用。