Detection method of chIRF3 fluorescent quantitation RT-PCR

• Main Authors: HUANG HAIPEI, LI SHANGQUAN, SHEN WEIWEI, CHEN BOWEN, CHEN YANYAN, HE XIUMIAO, LIU YUJING
• Format: Patent
• Language: Chinese; English
• Published: 17.09.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a detection method of chIRF3 fluorescent quantitation RT-PCR. The detection method comprises the following steps of performing primer design, performing total RNA extraction, performing chIRF3 gene amplification, enabling an amplification product to be connected with a pUC-T...