一种检测叶酸代谢相关基因多态性的方法

• Format: Patent
• Language: Chinese
• Published: 07.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

本发明提供了一种检测叶酸代谢相关基因多态性的方法，包括：预先合成MTHFR基因的C677T位点、A1298C位点和MTRR基因的A66G位点的特异性扩增引物与单碱基延伸引物；从待测样本中提取出待测样本基因组DNA，并作为DNA模板；配置包含特异性扩增引物和DNA模板的聚合酶链式反应PCR扩增体系；利用PCR仪对PCR扩增体系进行扩增；顺次进行消化处理；顺次将配置包含单碱基延伸引物的单碱基延伸反应体系加入反应体系中进行延伸反应，顺次进行脱盐纯化处理，获得纯化产物；利用包括有质谱仪的检测仪器检测纯化产物，确定待测样本的MTHFR基因的C677T位点、A1298C位点和MTRR基因的A66G位点的基因多态性。本方案能够提高患者体内叶酸代谢能力的检测效率。 The invention provides a method for detecting polymorphism of folate metabolism-related genes. The method comprises the steps that specific amplification primers and single base extension primers of the C677T site and A1298C site of an MTHFR gene and the A66G site of an MTRR gene are synthesized in advance; to-be-detected sample genomic DNA is extracted from a to-be-detected sample to serve as a DNA template; a polymerase chain reaction PCR amplification system containing the specific amplification primers and the DNA template is prepared; a PCR apparatus is used for amplifying the PCR amplification system; digestion treatment is performed in sequence; a single base extension reaction s