Hexosaminidase and coding gene related to strawberry softening, preparation and application thereof

• Main Authors: YIN HENG, WANG WENXIA, HE YANQIU, LIU TONGMEI
• Format: Patent
• Language: Chinese; English
• Published: 05.07.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT,VEGETABLES, EDIBLE SEEDS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THE PRESERVED, RIPENED, OR CANNED PRODUCTS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The present invention discloses two hexosaminidase gene sequences derived from strawberries (Fragaria x ananassa qing xia) and a preparation method and an application thereof, and a technical method using genetic engineering. The hexosaminidase gene is cloned into a pichia pastoris expression vector to obtain a pichia pastoris recombinant strain heterologously expressing hexosaminidase, abd the hexosaminidase prepared by the heterologous expression of the strain can uses p-nitrophenyl-N-acetyl-beta-D-amino glucosamine or p-nitrophenyl-N-acetyl-beta-D-aminogalactosamine as a substrate for reaction. The present invention also provides the application of the hexosaminidase in controlling postharvest fruit softening and preservative activity screening, and belongs to the field of fruit preservation. 本发明公开了两个来源于草莓(Fragaria×ananassa qing xiang)的氨基己糖苷酶基因序列及其制备方法和应用，即利用基因工程的技术方法，将该氨基己糖苷酶的基因克隆到毕赤酵母表达载体上，获得可异源表达该酶的毕赤酵母重组菌株，用该菌株异源表达制备的氨基己糖苷酶，能以p-硝基苯基-N-乙酰-β-D-氨基葡萄糖胺或P-硝基苯基-N-乙酰-β-D-氨基半乳糖胺为底物进行反应。本发明还提供了氨基己糖苷酶在控制采后果实软化及保