Italian penicillium mitochondria extraction method

• Main Authors: WANG YI, PENG LITAO
• Format: Patent
• Language: Chinese; English
• Published: 12.02.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an Italian penicillium mitochondria extraction method. A used extraction buffering solution is Hepes-KOH buffering solution with the pH of 7.4 and comprises 0.6M mannitol, 0.2percent BSA, 2mM EGTA and 2mM DTT. By utilizing an established Italian penicillium mitochondria extraction buffering system, the Italian penicillium mitochondria can be successfully extracted, the mitochondria are high in yield and good in activity, so that further research for the electron transfer chains, proteomics and genetic mechanism of the Italian penicillium mitochondria is possible, and the foundation is set for further researching the antibacterial mechanism of a sterilizing agent adopting the mitochondria as a target point for Italian penicillium. 本发明公开了种意大利青霉线粒体提取方法，所使用的提取缓冲液为pH为7.4的Hepes-KOH缓冲液，其中含有0.6M甘露醇、0.2％BSA、2mM EGTA、2mM DTT。本发明利用所建立的意大利青霉线粒体提取缓冲体系，成功的提取了意大利青霉线粒体，其中线粒体的产量较高，且活性较好，使得对意大利青霉线粒体的电子传递链、蛋白组学、遗传机制等的深入研究成为可能，从而为进步探究以线粒体为靶点的杀菌剂对意大利青霉的抑菌机理奠定了基础。