Preparation method and application of carbendazim hapten and antigen

• Main Authors: WU XIAOSHENG, FENG CAIWEI, CUI HUAPENG, PAN LINING, LU YAHUI, CHEN LI, LIU HUIMIN, FAN ZIYAN, TANG GANGLING, FAN MEIJUAN
• Format: Patent
• Language: Chinese; English
• Published: 25.01.2019
• Subjects: CHEMISTRY; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; TESTING

The invention discloses a preparation method and application of a carbendazim hapten and antigen. The method is characterized in that the carbendazim hapten is prepared from 2-aminobenzimidazole and butanedioic anhydride which are reacted; the carbendazim antigen is obtained by coupling the carbendazim hapten and carrier protein. The antigen prepared through the method presents a specific carbendazim antigenic determinantantigen epitope, so that screening out a high-specificity carbendazim monoclonal antibody becomes possible; the generated antibody is high in specificity and high in sensitivity, and can be used for building an enzyme-linked immunosorbent assay method and a colloidal gold test strip rapid determination method, so that carbendazim in tobacco and food is fast detected. 种多菌灵半抗原与抗原的制备方法及应用，其特征在于：所述多菌灵半抗原是由2-氨基苯并咪唑与丁二酸酐反应得到；所述多菌灵抗原是由多菌灵半抗原与载体蛋白偶联得到。本发明制备的抗原呈现出特异性的多菌灵抗原决定簇，使得筛选出高特异性的多菌灵单克隆抗体成为可能。产生的抗体特异性高、灵敏度高，可用于建立酶联免疫吸附测定方法和胶体金试纸快速测定法，从而实现烟草及食品中多菌灵的快速检测。