Ultra-low temperature preservation method of Chinese red pine pollen

• Main Authors: YAO RUILING, ZHANG YE, QIN ZIHAI, XIAO YUFEI, ZHANG XIAONING, CHEN BOWEN, LIU HAILONG
• Format: Patent
• Language: Chinese; English
• Published: 02.11.2018
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; FISHING; FORESTRY; HARVESTING; HUMAN NECESSITIES; HUNTING; MOWING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; TRAPPING

The invention discloses an ultra-low temperature preservation method of Chinese red pine pollen. The method comprises the steps of flower spike collection, pollen collection, pollen drying, pollen freezing preservation, pollen unfreezing, in vitro pollen germination and the like. The ultra-low temperature pollen preservation method is optimized aiming at the characteristics of the Chinese red pinepollen, and the method is simple and convenient, is simple in operation, prolongs the preservation time and the preservation life of the Chinese red pine pollen, improves the utilization ratio of theChinese red pine pollen and solves the problems of hybrid seed production such as blooming of flowers at different times of the Chinese red pine. 本发明公开了种马尾松花粉的超低温保存方法，其包括花穗棒的采集、花粉收集、花粉干燥、花粉冻存、花粉化冻、花粉离体萌发等步骤。该方法针对马尾松花粉特性对超低温花粉保存方法进行优化，方法简便，操作简单，提高马尾松花粉的保存期限和保存寿命，提升马尾松花粉利用率，解决马尾松花期不遇等杂交制种问题。