Detection method for genetic stability of hepatitis e virus and application

• Main Authors: MENG QINGXUE, HE JIA, QIN XIANGQIN, LIU QINGYU, YU MEILUN, LI YING, HAN WEILI, LI HAIYING, MEI XIAOXIAO, YUAN YUFENG, YAN ZENGFU
• Format: Patent
• Language: Chinese; English
• Published: 29.05.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a detection method for the genetic stability of hepatitis e virus and application and belongs to the field of biological engineering. The detection method comprises the following steps: recovering and culturing host bacterium liquid containing hepatitis e virus plasmids, and performing continuous multi-generational subculture; sampling at certain generation intervals and detecting; detecting the genetic stability of host bacteria which are manually preserved and contain the hepatitis e virus plasmids, so as to provide reliable support and guarantees for researches of thehepatitis e virus. The detection method provided by the invention is applied to hepatitis e virus culture, provides reference for the researches and culture of the hepatitis e virus and has importantapplication value. 本发明提供了种戊型肝炎病毒遗传稳定性的检测方法及应用，属于生物工程领域。本发明通过复苏培养含有戊型肝炎病毒质粒的宿主菌菌液，并进行连续多代的传代培养，间隔定的代数取样并检测，通过检测人工保藏的含有戊型肝炎病毒质粒的宿主菌的遗传稳定性，为研究戊型肝炎病毒提供可靠的支撑和保障；将上述检测方法应用到戊型肝炎病毒培养中，也可以为戊型肝炎病毒的研究和培养提供参考和借鉴，具有重要的应用价值。