Method for utilizing halohydrin dehalogenase engineering fungi to prepare R-phenyl glycidyl ether

• Main Authors: GAO JIAN, XUE FENG, YA XIANGJU, XIU YUANSONG, DING GE
• Format: Patent
• Language: Chinese; English
• Published: 06.04.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a method for utilizing halohydrin dehalogenase engineering fungi to prepare R-phenyl glycidyl ether, and relates to the field of preparing chiral drug midbody R-phenyl glycidylether. By means of the method for utilizing the halohydrin dehalogenase engineering fungi to prepare R-phenyl glycidyl ether, an engineering fungus thalli containing a halohydrin dehalogenas gene isused as a catalyst, a phenyl glycidyl ether substrate is catalyzed and converted to obtain R-phenyl glycidyl ether, and the method has high enantiomer selectivity and a very high catalysis activity and is environmentally friendly in the producing process. 本发明公开了种利用卤醇脱卤酶工程菌制备R-苯基缩水甘油醚的方法，涉及手性药物中间体R-苯基缩水甘油醚制备领域。本发明公开的利用卤醇脱卤酶工程菌制备R-苯基缩水甘油醚的方法，该方法以含有卤醇脱卤酶基因的工程菌菌体作为催化剂，催化苯基缩水甘油醚底物转化得到R-苯基缩水甘油醚，其具有良好的对映体选择性和很高的催化活力，生产过程中对环境友好。