Method of distinguishing mesenchymal stem cells and method of determining purity of mesenchymal stem cells

• Main Authors: MAO-KUANG DU, WEI-TING LIU, YUIH SU, CHANG-YO HSUAN, WILLIE LIN, MENG-WEI LI
• Format: Patent
• Language: Chinese; English
• Published: 13.03.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

A method of distinguishing mesenchymal stem cells (MSCs) from fibroblasts is provided. Also provided is a method of increasing a purity of mesenchymal stem cells (MSCs) population in a cell culture. The above-mentioned methods each comprise a step of sorting or isolating the cells by a marker of CD146 from a cell culture derived from a placenta-related tissue. Further provided is a method of assessing purity of mesenchymal stem cells (MSCs) in a cell culture derived from a placenta-related tissue, comprising determining the percentage of cells expressing CD146 in the culture. 本发明提供了种区别间充质干细胞的方法及测定间充质干细胞的纯度的方法。具体地，本发明提供种区别间充质干细胞(MSC)与纤维母细胞的方法。本发明还提供增加细胞培养物中的间充质干细胞(MSC)群体的纯度的方法。上述方法各自包含通过标记物CD146从源自胎盘相关组织的细胞培养物中分选或分离细胞的步骤。本发明还提供评估源自胎盘相关组织的细胞培养物中的间充质干细胞(MSC)的纯度的方法，包含测定该培养物中表达CD146的细胞的百分比。