SSR molecular marker primer capable of rapidly identifying robinia pseudoacacia polyploids

• Main Authors: WANG CUIYAN, LI HONGJING, XUN SHOUHUA, ZHANG YUANSHUAI, YANG QINGSHAN, SUN BAIYOU, MAO XIUHONG, YAN SHAOBO, LI SHUANGYUN
• Format: Patent
• Language: Chinese; English
• Published: 06.03.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an SSR molecular marker primer capable of rapidly identifying robinia pseudoacacia polyploids, wherein the primer base sequence is as shown in SEQ ID NO.1 and SEQ ID NO.2. According to the invention, robinia pseudoacacia polyploids can be rapidly identified from the molecular level without being affected by season, plant development stage and growing environment, so that reference and basis are provided for researches of rapid identification of robinia pseudoacacia polyploids, new variety protection, genetic diversity analysis evaluation and genetic breeding. By adopting the primer taken as an example in the invention, the detection rate of polyploids in 343 robinia pseudoacacia clones is up to 85.42%, thus sufficiently indicating high efficiency and applicability of the SSR primer, and verifying that multiple robinia pseudoacacia polyploids generating natural variation really exist in the natural world. 本发明公开了种快速鉴定刺槐多倍体的SSR分子标记引物，所述引物碱基序列如SEQ ID NO.1、SEQ ID NO.2所示。本发明实现了从分子水平快速鉴别刺槐多倍体