Improved CTAB (cetyltrimethylammonium bromide) method for extracting genomic DNA of cotton

• Main Authors: LUO XIAOLI, LI HUANLI, ZHU YONGHONG, XIA ZHI, LEI MENGLIN, YANG LIULIU, WU CUICUI, WEN SIYU, LI PENGBO, QIN LIXIA, CAO CAIRONG, PAN ZHUANXIA, DING XIAO
• Format: Patent
• Language: Chinese; English
• Published: 16.02.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an improved CTAB (cetyltrimethylammonium bromide) method for extracting genomic DNA of cotton. The method comprises the following steps: (1) grinding liquid nitrogen into powder, adding a CTAB buffer solution, and shaking the mixed solution in a water bath at 65 DEG C to obtain an extract solution I containing DNA; (2) performing cooling, adding RNase A, and performing a water bath at 37 DEG C; (3) adding equal volume of a solution A, uniformly mixing the solutions and performing centrifugation to obtain a supernatant I; (4) continuously adding equal volume of the solution A, uniformly mixing the solutions and performing centrifugation to obtain a supernatant II; (5) adding NaAc, then adding absolute ethyl alcohol, and performing shaking horizontally to form flocculent precipitates; (6) performing centrifugation, removing the supernatants, washing the precipitates, removing absolute ethyl alcohol, and performing drying to obtain precipitated DNA; (7) dissolvingthe precipitated DNA and