Extraction method, authentication primer and kit for DNA of pen shells

• Main Authors: GONG SHIHAI, DING MIN, GE CHAOWEI, LI JIARONG, WEI LEI, CHEN MAN, HE CHENG, YU WENCHAO, WANG XIAOTONG
• Format: Patent
• Language: Chinese; English
• Published: 26.12.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an extraction method, an authentication primer and a kit for DNA of pen shells. The extraction method comprises the steps of selecting a small amount of shell flesh (about 100mg) from abdomen edges of pen shells as an extraction sample, grinding shells, adding an EDTA solution for decalcifying shell powder, carrying out proteopepsis to release DNA, and adding leaching liquid to separate impurities such as proteins, polysaccharides and lipid from DNA, thus obtaining a DNA sample for molecular biology experiments and genetic breeding detection. The operation in the whole process is simple and rapid, extracted DNA is good in quality and can be preserved for a long time. The sampling part is the edge of the shell, so that an individual parent shell for breeding is not injured, and the foundation is laid for the molecular breeding of the shells, particularly the large-sized shells. 本发明公开了种江珧贝壳DNA提取方法、鉴定引物及试剂盒，该提取方法只选取江珧贝壳腹缘部100mg左右的少量贝壳作为提取样品，研碎贝壳后加入EDTA溶液对贝壳粉末进行脱钙，然后，进行蛋白消化，释放DNA，再加入抽提液将蛋白