Preparation method of multi-functional microorganism fish feed

• Main Authors: ZHANG CHEN, ZHU WEI, ZHU BEI
• Format: Patent
• Language: Chinese; English
• Published: 17.11.2017
• Subjects: CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; FERTILISERS; FERTILISERS CHARACTERISED BY THEIR FORM; FODDER; FOODS OR FOODSTUFFS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; MANUFACTURE THEREOF; METALLURGY; MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENTSUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVINGA SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES,SOIL-CONDITIONERS, WETTING AGENTS; PHOSPHATIC FERTILISERS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The invention relates to a preparation method of a multi-functional microorganism fish feed. The preparation method comprises the following steps of inoculating a liquid nutrient medium with saccharomyces cerevisiae containing recombinant bovine lactoferrin N-terminal peptide plasmid, performing shake culture for 10-24h, then inoculating a fresh culture medium with the cultured saccharomyces cerevisiae, when OD600 is 1.5 to 2.5, inoculating a fermenter with the saccharomyces cerevisiae, and performing fermentation for 60-72h; after the fermentation is finished, performing spray drying on fermentation liquor with a spray drying machine, so as to obtain bovine lactoferricin fermented powder; uniformly mixing fermented cottonseed powder with animal bone powder, corn leaf powder, hoof gelatine powder, shell powder, water and composite microorganisms to obtain a mixture, putting the mixed in a fermenting tank, performing fermentation to obtain a material A, placing the material A in a drying chamber, enabling the