Tri-segmented arenaviruses as vaccine vectors

• Main Authors: MERKLER DORON, KALLERT SANDRA MARGARETE, PINSCHEWER DANIEL DAVID, DARBRE ABDELRAHMAN STEPHANIE GABRIELLE, PAGE NICOLAS JEAN, KREUTZFELDT MARIO
• Format: Patent
• Language: Chinese; English
• Published: 29.09.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses tri-segmented arenaviruses as vaccine vectors. The present application relates to arenaviruses with rearrangements of their open reading frames ("ORF") in their genomes. In particular, described herein is a modified arenavirus genomic segment, wherein the arenavirus genomic segment is engineered to carry a viral ORF in a position other than the wild-type position of the ORF. Also described herein are trisegmented arenavirus particles comprising one L segment and two S segments or two L segments and one S segment. The arenavirus, described herein may be suitable for vaccines and/or treatment of diseases and/or for the use in immunotherapies. 本申请涉及在其基因组中具有开放阅读框("ORF")重排的沙粒病毒。特别地，本文描述的是修饰的沙粒病毒基因组片段，其中所述沙粒病毒基因组片段被工程化以在ORF的野生型位置之外的位置携带病毒ORF。本文还描述的是三片段沙粒病毒颗粒，其包含个L片段和两个S片段、或两个L片段和个S片段。本文描述的沙粒病毒可以适合于疫苗和/或治疗疾病和/或用于免疫治疗。