Modified PVPP/TiO2 nano composite material used for selectively separating cytochrome C in animal organs

• Main Authors: QI YUE, WANG JING, LIU CHUNLING, SUN KAI, GUI JINQIU, CHAO YUEGANG, CAI KERUI, SONG JIE, YAN LEI, WANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 30.06.2017
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OF MACROMOLECULAR COMPOUNDS; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SEPARATION; THEIR PREPARATION OR CHEMICAL WORKING-UP; TRANSPORTING; USE OF INORGANIC OR NON-MACROMOLECULAR ORGANIC SUBSTANCES ASCOMPOUNDING INGREDIENTS

The invention relates to a modified PVPP/TiO2 nano composite material used for selectively separating cytochrome C in animal organs, which relates to the technical field of biological medicine. The method comprises the following steps: 1) pre-treatment of nano TiO2 powder; 2) preparation of modified PVPP; and 3) preparation of modified PVPP/TiO2 nano composite material. The modified PVPP/TiO2 nano composite material is specially used for selectively separating cytochrome C in animal viscera, the selectivity can reach 85%, and the adsorption efficiency is as high as 89%, the elution efficiency of the adsorbed cytochrome C processed by a Na3PO4 eluent can reach 95%, so that the effective separation of cytochrome C in an acid extraction solution of animal organs is realized, and the yield of cytochrome C is greatly improved. 本发明公开了种动物脏器中细胞色素C选择性分离用改性PVPP/TiO纳米复合材料，涉及生物医药技术领域，具体制备方法包括如下步骤：(1)纳米TiO粉体预处理，(2)改性PVPP的制备，(3)改性PVPP/TiO纳米复合材料的制备。本发明所制改性PVPP/TiO纳米复合材料专用于动物脏器中细胞色素C的选择性分离，选择性达到85％，并且吸附效率高达89％，所吸附的细胞色素C经NaPO