Efficient identification and utilization method of transgenic insect-resistant cotton hybrid offspring germplasm

The invention discloses an efficient identification and utilization method of transgenic insect-resistant cotton hybrid offspring germplasm, comprising the following steps: (1) preparation of materials: transgenic cotton plants are obtained by meditation of agrobacterium tumefaciens, offspring seeds...

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Main Authors SUN ZHENGANG, ZHU YONGHONG, HU XIAOLI, XIA ZHI, PAN ZHUANXIA, ZHAO JUNXIA, CHEN GENG, GUO HAILI
Format Patent
LanguageChinese
English
Published 31.05.2017
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FERTILISING
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
PLANTING
SOWING
TRAPPING
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Summary:The invention discloses an efficient identification and utilization method of transgenic insect-resistant cotton hybrid offspring germplasm, comprising the following steps: (1) preparation of materials: transgenic cotton plants are obtained by meditation of agrobacterium tumefaciens, offspring seeds are obtained by hybridization, after depilation via sulfuric acid and sterilization treatment, the seeds are soaked into a container and the container is sealed and kept for 24h or longer at 20-30 DEG C, after the seeds are germinated, the seeds are inoculated after the seed coats are removed or the seeds are directly placed in a vessel used for cultivating the following preparations; and (2) inoculation and cultivation. The method in the invention is reasonable in design, the cotton transgenic technology and seed germination technology are combined organically, the transgenic insect-resistant cotton hybrid offspring germplasm is subject to identification and separation to sort out, thereby sorting out positive tr
Bibliography:Application Number: CN201611058764