Method for synthesizing 1,3-stearin-2-glyceryl monooleate through enzyme method

• Main Authors: CHEN YANG, WANG XINGGUO, WANG XIAOSAN, JIN QINGZHE, LU JIYUAN
• Format: Patent
• Language: Chinese; English
• Published: 08.03.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention discloses a method for synthesizing 1,3-stearin-2-glyceryl monooleate through an enzyme method. High oleic acid vegetable oil and a stearic acid acyl donor are mixed at a certain substrate molar ratio. A certain amount of lipase is added within or without a solvent system. The reaction is carried out for 1-10 hours at 60-80 DEG C and a reaction product containing SOS is obtained. The method of the invention inhibits an acyl transfer side reaction by using the enzyme specificity, efficiency and by controlling the reaction conditions. The reaction conversion rate is very high, at the same time, the synthesis time is shortened. It is easy to control the whole process. The reaction conditions are mild, and the cost can be greatly reduced through the recovery and utilization of the enzyme. It is convenient for large scale industrialization popularization and the application potential is huge. 本发明公开了种酶法合成1，3-二硬脂酸-2-油酸甘油酯的方法，将高油酸植物油和硬脂酸酰基供体以定的底物摩尔比混合，在溶剂体系或无溶剂体系下，加入定量的脂肪酶，在60～80℃下反应1～10h，得到含有SOS的反应产物。本