Linker peptide for constructing fusion protein

• Main Authors: DONG ZHAO, CHEN SI, LI ZIRUI, YANG LU, WANG ZHU, LI YUANLI, GAO YONGJUAN, SUN NAICHAO, LI QIANG, ZHENG YUNCHENG, MA XINLU
• Format: Patent
• Language: Chinese; English
• Published: 11.01.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a linker peptide for constructing a fusion protein, comprising a flexible peptide and a rigid peptide; the flexible peptide is composed of one or more flexible units, and the rigid peptide is composed of one or more rigid units, wherein each flexible unit comprises two or more amino acid residues selected from Gly, Ser, Ala and Thr, and each ridge unit comprises a plurality of glycosylation-sited carboxyl-terminal peptides (CTP) of human chorionic gonadotrophin beta-subunit. The linker peptide herein is more efficient in eliminating steric-hindrance effect between two fusion molecules, and reducing polymerization or activity decrease or loss due to misfolding or comformational change of active proteins; in addition, negatively-charged high-sialyl CTPs can resist removal by kidney, half-life period of the fusion molecules is further extended, bioavailability of the fusion protein is improved; more additionally, protection from glycosyl side chains of the CTPs enables reduced sensitivity