Simultaneous saccharification and co-fermentation of glucoamylase-expressing fungal strains with an ethanologen to produce alcohol from corn

• Main Authors: TEUNISSEN PAULA JOHANNA MARIA, SHETTY JAYARAMA K, REBOLI MATTHEW T, HUITINK JACQUELYN A, CLARKSON KATHLEEN A, CHOTANI GOPAL K
• Format: Patent
• Language: Chinese; English
• Published: 14.12.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

A conversion process provides using different co-cultured cell lines to express different sets of enzymes catalyzing the same process. For example, in a Simultaneous Saccharification and Co-Fermentation (SSCF) process, a starch substrate is converted to alcohol by contacting the substrate with yeast and Aspergillus niger cells. Because A. niger expresses an endogenous glucoamylase and alpha-amylase, these enzymes do not need to be added during the SSCF process. 本发明题为"表达葡糖淀粉酶的真菌菌株和产乙醇物的同时糖化和共发酵以由玉米产生醇"。本发明公开了种转化方法，所述转化方法提供了用不同的共培养细胞系表达催化该转化方法的不同组的酶。例如，在同时糖化和共发酵(SSCF)过程中，通过使底物与酵母和黑曲霉细胞接触来使淀粉底物转化为醇。因为黑曲霉表达内源性葡糖淀粉酶和α-淀粉酶，所以在SSCF过程期间中不需要添加这些酶。