Preparation method of silkworm chrysalis small peptide

• Main Authors: ZHANG BIN, ZHANG SHIWEI, TAO SHAOQIANG, LI YUFENG
• Format: Patent
• Language: Chinese; English
• Published: 07.12.2016
• Subjects: FOODS OR FOODSTUFFS; HUMAN NECESSITIES; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS; PROTEIN COMPOSITIONS FOR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; WORKING-UP PROTEINS FOR FOODSTUFFS

The invention discloses a preparation method of silkworm chrysalis small peptide. The invention performs control optimization from the aspects of crushing and sieving mesh, adding amount, enzymolysis temperature, pH, enzyme dosage and the like, the adding amount of pepsin and trypsin is small, the cost is saved, at the same time, the bionic enzymolysis involved in the invention has high yield, pepsin and trypsin combination is utilized to conduct bionic enzymolysis, and the extract yield is 1.5-2 times that of the traditional process. In addition, the method provided by the invention utilizes a combined technology of ultrafiltration membrane separation, electrodialysis and resin column chromatography to conduct separation and purification on the bionic enzymolysis peptide, the loss of active peptide is less than 2%, and the small molecule peptide with a relative molecular mass of less than 2KD is greater than 84%. The method reduces the loss of active peptide obtained by enzymolysis, at the same time, the con