Tissue culture and rapid propagation method for macadimia nuts

The invention discloses a tissue culture and rapid propagation method for macadimia nuts. The tissue culture and rapid propagation method for the macadimia nuts comprises the steps that stem sections with buds and with the length of 4 cm are cut out of mature macadimia nut trees to serve as explants...

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Bibliographic Details
Main Author LING HUABIAO
Format Patent
LanguageChinese
English
Published 16.03.2016
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Summary:The invention discloses a tissue culture and rapid propagation method for macadimia nuts. The tissue culture and rapid propagation method for the macadimia nuts comprises the steps that stem sections with buds and with the length of 4 cm are cut out of mature macadimia nut trees to serve as explants, and the explants are subjected to disinfection treatment; the obtained explants undergo induced culture for 20-24 days, wherein the temperature is 23-25 DEG C, the light intensity is 2000-2200 Lux, and the lighting time is 13-15 hours every day; cultivated newly-grown axillary bud seedlings are cut into the stem sections under the aseptic conditions, and each stem section is only with one axillary bud and is planted in a rooting medium to be cultivated at the temperature of 25-27 DEG C. The tissue culture and rapid propagation method for the macadimia nuts sequentially comprises three stages: at the first stage, cultivation is performed for 12-14 days under the conditions of the light intensity of 2200-2400 Lux a
Bibliography:Application Number: CN20151868889