Liquid-stage chip constant-temperature detection method for tiny RNA

• Main Authors: FU WEILING, XIA HAN, HUANG JUNFU, HUANG QING
• Format: Patent
• Language: English
• Published: 28.10.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a liquid-stage chip constant-temperature index amplification and detection method for tiny RNA target molecules. By fixing upstream and/or downstream primers to the surfaces of encoding microspheres and marking the two sides of notch agent recognition sequences of the primers fixed to the surfaces of the encoding microspheres with fluorescence report molecules which have the fluorescence resonance energy transfer effect and cancellation molecules, finally the high-throughput detection of various target molecules is achieved through target molecule specificity detectable fluorescence signals on the surfaces of the encoding microspheres. The design difficulty of miRNA inherent attributes on the amplification detection method for the target molecules is lowered, a liquid-state constant-temperature index amplification and detection system based on oligonucleotides primers is provided, and the method for achieving constant-temperature index amplification and absolute quantification under the constant-temperature condition can be achieved.