Method for identifying tobacco raw materials by applying FMYYSSR-2 marker

• Main Authors: HOU HONGWEI, FENG XIAOMIN, LIU NAN, LUO ANNA, HE SHENGBAO, ZHANG WEI, XING JUN, HU QINGYUAN, WANG YINGYUAN
• Format: Patent
• Language: English
• Published: 07.10.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a method for identifying tobacco raw materials by applying FMYYSSR-2 molecular marker technology, belonging to the field of molecular biology study. Whether samples are tobaccos is identified by applying an FMYYSSR-2 marker after the gene data information of the tobaccos is screened. The method is characterized in that after DNA (deoxyribonucleic acid) of each sample is extracted by a CTAB (cetyl trimethyl ammonium bromide) method, PCR (polymerase chain reaction) amplification is carried out on DNA by using designed primers, and after electrophoretic analysis, the specific band positions of tobacco species are determined; whether the samples are tobaccos is judged according to whether amplified bands are generated in the corresponding positions of the samples to be detected. As the specificity of DNA of the tobacco species is used as the identification basis, compared with traditional identification methods, the method disclosed by the invention has the advantages that the method is more objective, more accurate and more reliable.