Lymantria dispar linnaeus heatshock protein Hsp23 gene and application of dsRNA thereof in nuisanceless control

• Main Authors: ZOU CHUANSHAN, SUN LILI, LIU PENG, WANG CHAO, CAO CHUANWANG
• Format: Patent
• Language: English
• Published: 29.04.2015
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a lymantria dispar linnaeus heatshock protein Hsp23 gene and application of dsRNA thereof in nuisanceless control. A nucleotide sequence and a coded amino acid sequence of the Hsp23 gene are shown by SEQ ID No.1, the dsRNA is synthesized from part of sequence fragments of the gene, and the sequence of the dsRNA is shown by SEQ ID No.2. The dsRNA of the Hsp23 gene can be applied to control of the growth and development of lymantria dispar linnaeus. An experimental result shows that after the dsRNA of the Hsp23 gene is injected into a lymantria dispar linnaeus larva, compared with a contrast (injected with ddH2O and GFP gene dsRNA), a relatively strong silencing effect is achieved, so that the growth and development of the lymantria dispar linnaeus larva are inhibited. Therefore, the invention provides a silencing technology capable of using Hsp23 to control an important forest pest, namely the lymantria dispar linnaeus and lepidopterous pests with similar structural domains, thereby providing a new train of thought and a new technology for green control of forest pests.