High-efficiency enrichment technology for isothiocyanate in brassica bud seedlings

The invention relates to a high-efficiency enrichment technology for isothiocyanate in brassica bud seedlings. The technology is characterized by comprising the following steps: in 5 to 10 d after germination of brassica seeds, subjecting the seeds to heat shock at 40 to 70 DEG C for 10 to 20 min on...

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Bibliographic Details
Main Authors GUO LIPING, GUO QIANGHUI, GU ZHENXIN, YANG RUNQIANG, WANG ZHIYING
Format Patent
LanguageChinese
English
Published 05.11.2014
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a high-efficiency enrichment technology for isothiocyanate in brassica bud seedlings. The technology is characterized by comprising the following steps: in 5 to 10 d after germination of brassica seeds, subjecting the seeds to heat shock at 40 to 70 DEG C for 10 to 20 min once each day; after harvesting of bud seedlings, grinding and homogenizing the harvested bud seedlings with distilled water according to a material-liquid ratio of 1: 5-10 (w/v), carrying out incubation at 37 DEG C for 3 h and allowing endogenous glucosinolate (thioglycoside) to be thoroughly hydrolyzed so as to produce isothiocyanate with an anticancer effect; and extracting isothiocyanate in a hydrolysate with dichloromethane and carrying out centrifugation so as to prepare an extract rich in isothiocyanate. The technology has the advantages of a wide application scope, simple operation and low production cost. After the brassica bud seedlings have undergone heat shock and homogenization, the content of produced i
Bibliography:Application Number: CN2014118040