Cereus sinensis verrill soluble cell peptide CCT-I and separating and purifying method and application thereof

• Main Authors: WAN XIHE, WANG LIBAO, CUI HONGLI, ZHANG ZHAOHUI, YANG HAIPING, QIN SONG, WU JIANPING, SHI WENJUN
• Format: Patent
• Language: Chinese; English
• Published: 27.11.2013
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention discloses a cereus sinensis verrill soluble cell peptide CCT-I and a separating and purifying method and an application thereof. The separating and purifying method comprises the following steps: after cereus sinensis verrill tentacle crude toxin is salted out and fractionally precipitated with ammonium sulfate, active sites are separated and purified through Sephadex G-100 and superdex 75 gel filtration chromatography columns in sequence, and the soluble cell peptide is obtained through separation from a cereus sinensis verrill tentacle at the first time and is finally authenticated as the novel cereus sinensis verrill soluble cell peptide that has not been reported through SDS-PAGE and LC-MS, wherein two peptides (alpha and beta) in the novel cereus sinensis verrill soluble cell peptide are similar in molecular weight of about 80 kDa, and the HPLC test shows that the two peptides are high in purify and similar in polarity. The half soluble cell rate is tested to be 5.75 micrograms*mL<-1>, and