Key technology for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province

• Main Authors: LIN YU, PENG BIAO, PENG DONGHUI, RUAN YIHUAN, HE BIZHU, HE GUANRONG, RUAN YIYAO
• Format: Patent
• Language: Chinese; English
• Published: 11.09.2013
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention provides a key technology for culturing in vitro embryos and regenerating plants of ormosia hosiei in western Hubei province, belonging to the technologies of preserving and massively propagating an ormosia hosiei seed resource. The problems that the ormosia hosiei seeds are difficult to obtain and easily eaten by rats, since the seeds are hard, the dried seed husks can not absorb water easily due to poor water permeability, the natural propagation capability and the spreading capability are not strong, the fructification age is late, natural regeneration is difficult, blooming and fructification require 3-5 years, and good seedlings are scarce in the prior art are solved. According to the key technology, full seeds fruiting in the current year are selected as a material; after 20-35 days of embryo induction cultivation, robustly growing embryo plants can sprout up; after 25-45 days of multiplication cultivation, 20-35 days of strong seedling cultivation and 25-35 days of rooting cultivation, se