Xylosidase Xyl43B with high xylose tolerance, and gene and application thereof

• Main Authors: WANG XINGJI, GUO QINGWEN, ZHANG JIE, LIU WENLONG
• Format: Patent
• Language: Chinese; English
• Published: 04.09.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of the genetic engineering, and in particular relates to xylosidase Xyl43B with high xylose tolerance, and a gene and an application thereof. The invention provides xylosidase Xyl43B which is from humicola sp.L8, and an amino acid sequence of xylosidase Xyl43B is shown as SEQ ID No.1. The invention provides a coding gene xyl43B for coding xylosidase. The xylosidase has the following properties that the xylose tolerance is high (ki value is 292mM), the xylosidase is stable at the preferable condition that pH is 7.0 and between 5.5 and pH 10.0, and the preferable temperature is 50DEG C and xylosidase has good thermal stability at the temperature of 50DEG C. As a novel zymin, the xylosidase can be widely applied to the food, feed and energy industries.