Annual slide preparation method of plum blossom chromosome

• Main Authors: ZHANG QIXIANG, CHEN JINGXIN, YANG WEIRU, SUN LIDAN
• Format: Patent
• Language: Chinese; English
• Published: 07.11.2012
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides an annual slide preparation method of plum blossom chromosome. According to the method, the following meristems are selected as materials for preparing the slide according to time: 1) spring: new stem tips of branches in early spring; 2) early summer: immature embryos of plum blossom and growth points obtained from tissue culture of immature embryos; 3) summer and autumn: top growth points of annual seedlings of plum blossom; and 4) winter: stem tips obtained from hydroponics of dormant branches of plum blossom. The above materials for preparing the slide are treated with chromosome slide preparation, which comprises successive steps of low temperature fixing, enzyme and dyeing tabletting, to obtain a clear chromosome image with good dispersion. The method provided by the invention solves problems of short material collection cycle and time restriction of chromosome slide preparation in the study course of plum blossom chromosome.