Dual-fluorescence plasmid, dual-fluorescence plasmid-Based method for analyzing deoxyribonucleic acid (DNA) mismatch repair functional activity in living cell and application thereof

• Main Authors: REN XIANGPENG, BAI CHENGLIAN, DONG QIAOXIANG, HUANG ZHANGJIANG, CHEN YUANHONG, GE HONGSHAN
• Format: Patent
• Language: Chinese; English
• Published: 13.07.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a dual-fluorescence plasmid carrying enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) genes, wherein the EGFP and RFP genes are positioned in the same reading frame. The constructed dual-fluorescence plasmid can realize co-expression of two fluorescence proteins by placing two fluorescence protein genes in the same reading frame. Meanwhile, the dual-fluorescence plasmid is applied to deoxyribonucleic acid (DNA) mismatch repair functional activity analysis in a living cell to improve the verification accuracy at unicellular level, and the method is simple and has high sensitivity of indicating parameters and high accuracy. Therefore, the invention provides a novel effective detecting method for DNA mismatch repair functional activity detection in the living cell. The invention also provides application of the dual-fluorescence plasmid in preparing reagent for detecting the DNA mismatch repair functional activity in the living cell.