Method for creating new high oleic acid germplasm by treating rape germinating seeds with low-dose Co-gamma rays

• Main Authors: ZHANG JIEFU, CHEN XINJUN, FU SHOUZHONG, PU HUIMING, HU MAOLONG, GU HUI, CHEN SONG, FU SANXIONG, LONG WEIHUA, CHEN FENG, GAO JIANQIN, QI CUNKOU
• Format: Patent
• Language: Chinese; English
• Published: 07.11.2012
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FERTILISING; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PLANTING; SOWING; TRAPPING

The invention discloses a method for creating a new high oleic acid germplasm by treating rape germinating seeds with low-dose Co-gamma rays, and belongs to a plant breeding method. The method comprises the following steps: firstly placing dried seeds of common rape in an illuminating incubator at 25 DEG C for germination treatment; carrying out low-dose radiation with the Co-gamma rays after seed radicle germination, wherein, the radiation dose is 80Gray; preserving the radiation seeds in the illuminating incubator at 25 DEG C; and planting buds in a field after plumules grow to about 1cm. In the method, the oleic acid content of radiation progenies is directionally selected by a bulk method of breeding, and a plant N1379T with high oleic acid content up to 85% is obtained through positive breeding of continuous five generations.