Primary culture method for pancreas acinar cells

The invention discloses a primary culture method for pancreas acinar cells. The primary culture method for the pancreas acinar cells comprises the following steps of: (1) preparing pancreas acinar cell suspension; and (2) separating and purifying the pancreas acinar cells by a Percoll discontinuous...

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Bibliographic Details
Main Authors LONG YOUMING, CHEN JINGHUA, GE QUANXING, WANG HUI, CHEN KEN, CUI SHULAN
Format Patent
LanguageChinese
English
Published 03.10.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a primary culture method for pancreas acinar cells. The primary culture method for the pancreas acinar cells comprises the following steps of: (1) preparing pancreas acinar cell suspension; and (2) separating and purifying the pancreas acinar cells by a Percoll discontinuous density gradient precipitation method. The method can successfully culture the pancreas acinar cells, smoothly perform passage and lay a foundation for pathogenesis or pharmacotherapy of related diseases of the pancreas acinar cells; and the activity of the cells reaches over 96 percent.
Bibliography:Application Number: CN20101212144