Generation bacterium of ring dextrin glucosyl transferase

• Main Authors: HUANG ZUNXI, MENG YANFEN, XU BO, TANG XIANGHUA
• Format: Patent
• Language: Chinese; English
• Published: 27.02.2008
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a manufactured bacterium of annular dextrin glucosyltransferase, which is characterized by the following: obtaining the separated strain LZ-10 as Bacillus sp with preservation number at CGMCC No. 2096; displaying the fittest reacting pH value of annular dextrin glucosyltransferase at 9. 0 and the fittest reacting temperature at 65 deg. c with good thermal stability; insulating at 60-65 deg. c over 30min to keep residual enzymatic activity more than 95%; insulating with pH value at 8. 5-9. 5 for 30 min to keep the residual enzymatic activity more than 90%. The enzyme is compatible with thermostability and alkaliresistant, which is fit for enzymatically industrial manufacturing course of annular dextrin.