Anticuerpos de dominio simple vhh contra el virus sars-cov-2

• Main Authors: Teresa Pinto, Alexei Cuevas, Ananda Muller, Alejandro Rojas, Constanza Salinas, Natalia López, Benjamín Uberti, Ronald Jara, Zaray Miranda, Guillermo Valenzuela, Johanna Himelreichs, Yorka Cheuquemilla
• Format: Patent
• Language: Spanish
• Published: 07.07.2023
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

La invención proporciona un conjunto de nuevos anticuerpos de dominio simple VHH contra SARS-CoV-2 (SEQ ID N°1-6) y su uso para detectar y neutralizar el virus de tipo silvestre. One-step rapid gradient method for rapid generation of nanoantibodies (HHV) against any antigen. Where the method comprises the separation of nanoantibodies against a specific antigen from a nanoantibody expression library through the following steps:(a) binding the antigen of interest to beads made of protein-binding polymers(b) incubating the microorganisms of the expression library with the pearls of stage (a),(c) placing the beads of step (b) in a tube with an inert medium with a density greater than or equal to 1 g/mL and centrifuge for 45 s to 2 minutes at a rate between 150 and 250 g;d) discarding the upper fraction and supernatant with the free microorganisms, and obtain the beads with the linked microorganisms, which correspond to those that express HHV that recognizes the antigen.