MIKROBIOLOGISCHES VERFAHREN ZUR HERSTELLUNG VON SEKUNDAEREN GLYKOSIDEN AUS PRIMAEREN DIGITALISGLYKOSIDEN

• Main Authors: ATTILA SZENTIRMAI, MARIA TRINN, GEZA WACK, ZOLTAN SZELECZKY, ISTVAN ZAMBO, GABRIELLA MURANYI-FARKAS, ERNOE SZARKA, ENDRE TOEMOERKENY, LAJOS ILA, KATALIN SZERECZ-VOELCSEY, TIBOR BALOGH, ERZSEBET ZSOKA-SOMKUTI, LASZLO TOELGYRSI, EMMA SIMONOVITS
• Format: Patent
• Language: German
• Published: 15.08.1984
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; SPIRITS; STEROIDS; VINEGAR; WINE

A process for the preparation of secondary digitalis glycosides of general formula (I), (wherein R1 and R2, which may be the same or different, each represents a hydrogen atom or a hydroxy group) comprises fermenting a primary glycoside of formula (II), (wherein R1 and R2are as defined above, R3 represents a hydrogen atom or an acetyl group and R4 represents a hydrogen atom or a D-glucose unit, with the proviso that at least one of R3 and R4 is other than a hydrogen atom) with a culture of a Streptomyces griseolus strain deposited at the Hungarian National Collection of Microorganisms (Budapest, Hungary) on 6th December 1977 under No. MNG 168 or a mutant thereof or with an enzyme preparation produced therefrom and separating the product of formula (I) thus obtained from the fermentation broth.