METHODS, AND SYSTEMS TO DETECT TRANSPLANT REJECTION
This application provides methods and systems for determining transplant status. In some embodiments, the method comprises obtaining a biological sample from an organ transplant recipient who has received an organ; isolating cell-free nucleic acids from the biological sample; measuring the amount of...
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Format | Patent |
Language | English French |
Published |
12.03.2020
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Abstract | This application provides methods and systems for determining transplant status. In some embodiments, the method comprises obtaining a biological sample from an organ transplant recipient who has received an organ; isolating cell-free nucleic acids from the biological sample; measuring the amount of each allele of one or more polymorphic nucleic acid targets in the biological sample; identifying the donor specific allele using a computer algorithm based on the measurements of the one or more polymorphic nucleic acid targets, whereby detecting one or more donor-specific circulating cell-free nucleic acids, detecting tissue injury based on the presence or amount of said one or more donor-specific nucleic acids, thereby determining transplant status.
L'invention concerne des procédés et des systèmes pour déterminer l'état d'une greffe. Dans certains modes de réalisation, le procédé comprend l'obtention d'un échantillon biologique d'un receveur de greffe d'organe qui a reçu un organe ; l'isolement des acides nucléiques acellulaires de l'échantillon biologique ; la mesure de la quantité de chaque allèle d'une ou de plusieurs cibles d'acides nucléiques polymorphes dans l'échantillon biologique ; l'identification de l'allèle spécifique donneur à l'aide d'un algorithme informatique sur la base des mesures de la cible ou des cibles d'acides nucléiques polymorphes, ce qui permet de détecter un ou plusieurs acides nucléiques acellulaires circulants spécifiques au donneur, de détecter une lésion tissulaire sur la base de la présence ou de la quantité dudit ou desdits acides nucléiques spécifiques au donneur, ce qui permet de déterminer l'état d'une greffe. |
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AbstractList | This application provides methods and systems for determining transplant status. In some embodiments, the method comprises obtaining a biological sample from an organ transplant recipient who has received an organ; isolating cell-free nucleic acids from the biological sample; measuring the amount of each allele of one or more polymorphic nucleic acid targets in the biological sample; identifying the donor specific allele using a computer algorithm based on the measurements of the one or more polymorphic nucleic acid targets, whereby detecting one or more donor-specific circulating cell-free nucleic acids, detecting tissue injury based on the presence or amount of said one or more donor-specific nucleic acids, thereby determining transplant status.
L'invention concerne des procédés et des systèmes pour déterminer l'état d'une greffe. Dans certains modes de réalisation, le procédé comprend l'obtention d'un échantillon biologique d'un receveur de greffe d'organe qui a reçu un organe ; l'isolement des acides nucléiques acellulaires de l'échantillon biologique ; la mesure de la quantité de chaque allèle d'une ou de plusieurs cibles d'acides nucléiques polymorphes dans l'échantillon biologique ; l'identification de l'allèle spécifique donneur à l'aide d'un algorithme informatique sur la base des mesures de la cible ou des cibles d'acides nucléiques polymorphes, ce qui permet de détecter un ou plusieurs acides nucléiques acellulaires circulants spécifiques au donneur, de détecter une lésion tissulaire sur la base de la présence ou de la quantité dudit ou desdits acides nucléiques spécifiques au donneur, ce qui permet de déterminer l'état d'une greffe. |
Author | TYNAN, JOHN ALLEN LEFKOWITZ, ROY BRIAN XU, CHEN |
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Discipline | Medicine Chemistry Sciences Physics |
DocumentTitleAlternate | PROCEDES ET SYSTEMES POUR DETECTER UN REJET DE GREFFE |
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Notes | Application Number: CA20193107467 |
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Snippet | This application provides methods and systems for determining transplant status. In some embodiments, the method comprises obtaining a biological sample from... |
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SubjectTerms | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATIONTECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING ORPROCESSING OF MEDICAL OR HEALTHCARE DATA INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
Title | METHODS, AND SYSTEMS TO DETECT TRANSPLANT REJECTION |
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