SINGLE CELL MICROFLUIDIC DEVICE
Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluid...
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Format | Patent |
Language | English French |
Published |
20.09.2022
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Abstract | Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.
L'invention concerne des procédés d'obtention, de manière non destructive, du génotype d'une cellule végétale provenant d'une plante échantillon. La cellule végétale est isolée d'un échantillon de cellules végétales à l'aide d'un dispositif microfluidique intégré. Le dispositif microfluidique intégré comprend un piège à cellule individuelle situé en aval d'un canal microfluidique, le dispositif microfluidique étant conçu pour piéger la cellule végétale dans le piège à cellule individuelle. L'ARN est extrait de la cellule végétale en mettant en contact ladite cellule végétale avec une sonde de microscope à force atomique (AFM) et en attirant l'ARNm du locus d'intérêt vers l'extrémité de la sonde à l'aide d'une force de diélectrophorèse DEP sous le champ de courant alternatif (c.a.) appliqué à la sonde. Dans un autre mode de réalisation, l'ADNc est analysé pour déterminer des profils d'expression génique de la cellule végétale. Dans un autre mode de réalisation, l'ARNm est analysé pour déterminer des profils d'expression génique de la cellule végétale. |
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AbstractList | Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.
L'invention concerne des procédés d'obtention, de manière non destructive, du génotype d'une cellule végétale provenant d'une plante échantillon. La cellule végétale est isolée d'un échantillon de cellules végétales à l'aide d'un dispositif microfluidique intégré. Le dispositif microfluidique intégré comprend un piège à cellule individuelle situé en aval d'un canal microfluidique, le dispositif microfluidique étant conçu pour piéger la cellule végétale dans le piège à cellule individuelle. L'ARN est extrait de la cellule végétale en mettant en contact ladite cellule végétale avec une sonde de microscope à force atomique (AFM) et en attirant l'ARNm du locus d'intérêt vers l'extrémité de la sonde à l'aide d'une force de diélectrophorèse DEP sous le champ de courant alternatif (c.a.) appliqué à la sonde. Dans un autre mode de réalisation, l'ADNc est analysé pour déterminer des profils d'expression génique de la cellule végétale. Dans un autre mode de réalisation, l'ARNm est analysé pour déterminer des profils d'expression génique de la cellule végétale. |
Author | LEE, ABRAHAM P YUN, YUE LEE, DO-HYUN TAO, YINGLEI WICKRAMASINGHE, H. KUMAR LI, XUAN |
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Snippet | Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample... |
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SubjectTerms | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROMSOLID MATERIALS OR FLUIDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS SPIRITS TRANSPORTING VINEGAR WINE |
Title | SINGLE CELL MICROFLUIDIC DEVICE |
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