CONJUNTO DE OBTENCAO E DEPOSICAO DE CELULAS ENDOTELIAIS E PROCESSO PARA OBTER E DEPOSITAR DE MANEIRA ESTERIL CELULAS ENDOTELIAIS SOBRE UM ENXERTO A SER IMPLANTADO EM UM PACIENTE

• Main Authors: TONY A. CUTSHALL, BRUCE E. JARRELL, CHRISTOPHER J. BROOKS, DEBORAH G. ROSE, PAUL G. ALCHAS, FRANK A. AUGELLO, JONATHAN B. GABEL, WES PRAIS, JOSEPH A. DIPISA, JR, PAUL J. MULHAUSER, STUART K. WILLIAMS
• Format: Patent
• Language: Portuguese
• Published: 06.08.1991
• Edition: 5
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BANDAGES, DRESSINGS OR ABSORBENT PADS; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF,TUBULAR STRUCTURES OF THE BODY, E.G. STENTS; DIAGNOSIS; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; FILTERS IMPLANTABLE INTO BLOOD VESSELS; FIRST-AID KITS; FISHING; FOMENTATION; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; IDENTIFICATION; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROSTHESES; SURGERY; TRAPPING; TREATMENT OR PROTECTION OF EYES OR EARS

The invention is an endothelial cell procurement and deposition kit for collecting fat from a patient, processing said fat to produce an endothelial cell deposition product, and depositing said product on the surface of a graft, all under sterile conditions established and maintained within the components of said kit comprised of: fat collection means for collecting subcutaneous fat from a patient; digestion means connectable to said fat collection means to maintain sterility during reception of said fat and for retaining said fat under sterile conditions during rinsing and digestion to produce a digested product; endothelial cell isolation means connectable to said digestion means for maintaining sterile conditions during reception of said digested product and for separating and isolating microvessel endothelial cells from said digested product to produce an endothelial cell product; cell deposition means connectable to said isolation means for maintaining sterile conditions during reception of said endothelial cell product and for depositing said cells on the surface of a graft to be implanted in a patient and facilitating implantation of said endothelial graft into a patient.