método para detectar especificamente a presença ou ausência de uma ou mais mutações de nucleotídeos simples em um ou mais genes ou proteínas de hiv-1, método para detectar a presença ou ausência de mutações, primer de oligonucleotídeo seletivo, oligonucleotídeo, kit para detectar uma mutação, mistura de oligonucleotídeos para a detecção seletiva de mutações e processo de detecção simultânea de duas ou mais mutações em uma ou mais proteínas de hiv-1

• Main Authors: JEFFREY A. JOHNSON, JONATHAN T. LIPSCOMB, XIERONG WEI, WALID M. HENEINE
• Format: Patent
• Language: Portuguese
• Published: 26.09.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase, protease, or integrase of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS: 1-89, 96-122, and 124-151. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS: 1-89, 96-122, and 124-151. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV simultaneously or sequentially. Mutations in the reverse transcriptase, protease, or integrase of HIV can be detected using the methods described herein.