Short oligonucleotides for the inhibition of vegf expression

• Main Authors: EUGEN UHLMANN, RICHARD WOESSNER, ALAN BITONTI, ANUSCHIRWAN PEYMAN
• Format: Patent
• Language: English
• Published: 28.02.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; SUGARS; VINEGAR; WINE

The present invention relates to a short oligonucleotide or a derivative thereof which has a sequence that corresponds to a particular part of a nucleic acid sequence which encodes VEGF (vascular endothelial growth factor) and which has a length of maximum 15 nucleotides, the invention further relates to a method of making the oligonucleotide and the use thereof. A short oligonucleotide or a derivative thereof, which has a length of 10 to 15 nucleotides and which corresponds to a part of a VEGF encoding sequence, wherein the part of the VEGF encoding sequence to which the oligonucleotide corresponds to has one of the sequences SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5 or SEQ ID NO. 6 or a part thereof, wherein SEQ ID NO. 1 is 5'- CCCGGCCCCGGTCGGGCCTCCG - 3', SEQ ID NO. 2 is 5'- CGGGCCTCCGAAACC -3' , SEQ ID NO. 3 is 5'- GCTCTACCTCCACCATGCCAA -3', SEQ ID NO. 4 is 5'- GTGGTCCCAGGCTGCACCCATGGC -3', SEQ ID NO. 5 is 5'- CATCTTCAAGCCATCC -3', and SEQ ID NO. 6 is 5'- TGCGGGGGCTGCTGC -3'.