Method for in vitro culture of ovarian follicles

The present invention is related to a method for in vitro culture of mammalian ovarian follicles, comprising the following subsequent steps: providing a suitable container for the in vitro culture, selecting a follicle from an ovary of a mammal, said follicle comprising at least a theca or pre-theca...

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Bibliographic Details
Main Authors JOHAN SMITZ, RITA CORTVRINDT
Format Patent
LanguageEnglish
Published 24.06.2002
Edition7
Subjects
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Summary:The present invention is related to a method for in vitro culture of mammalian ovarian follicles, comprising the following subsequent steps: providing a suitable container for the in vitro culture, selecting a follicle from an ovary of a mammal, said follicle comprising at least a theca or pre-theca cell, a granulosa cell and an oocyte, an optional first culture step using an attachment prohibiting first medium free from oil, a second culture step using an attachment promoting second medium free from oil arranged to, and the retrieval of the matured follicle.
Bibliography:Application Number: AU20240066902