Methods And Compositions For Increasing Efficiency Of Targeted Gene Modification Using Oligonucleotide-Mediated Gene

• Main Authors: BEETHAM, Peter R, SCHOPKE, Christian, PEARCE, James, SEGAMI, Rosa E, MOZORUK, Jerry, GOCAL, Gregory F. W, SAUER, Noel Joy
• Format: Patent
• Language: English
• Published: 03.09.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Abstract The invention provides improved methods for the modification of genes in plant cells, and plants and seeds derived therefrom. More specifically, the invention relates to the increased efficiency of targeted gene mutation by combining gene repair oligonucleotides with approaches that enhance...