Method for propagating a virus

• Main Authors: RIMSTAD, ESPEN, FINSTAD, OYSTEIN W, DAHLE, MARIA K
• Format: Patent
• Language: English
• Published: 24.03.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present disclosure relates to the field of aquaculture, farmed fish and the occurrence of disease in farmed fish. More particularly, the present disclosure provides means for preparing large amounts of an orthoreovirus, such as piscine reovirus (PRV), by ex vivo propagation in nucleated erythrocytes. The present document also provides means for preparing vaccine compositions comprising orthoreovirus.